Immunofluorescent Assay of De Novo Vimentin Expression in Ovarian Cancer Tissues: Surgical Specimens vs Paraffin Embedded Tissue Blocks

The results of epithelial-mesenchymal transition analysis in serous ovarian cancer by the developed method of double immunofluorescent staining and flow cytometry while surgical tumor specimens and paraffin embedded tissue blocks are compared. When estimating de novo expression of the mesenchymal protein vimentin in epithelial tumor cells expressing cytokeratins, the results turn out to be close not only in quantitative parameters but also visually in the nature of cell distribution on the dotted plots. The results obtained indicate that the introduction of the developed new technology for the molecular diagnostics of the epithelial-mesenchymal transition of human tumors into routine laboratory practice is promising.