Expression of vasopressin V2 receptor in Xenopuslaevis oocytes by porcine kidney cell line (LLC-PK1) messenger RNA

Abstract Vasopressin V2 receptor was expressed in Xenopus laevis oocytes which were injected with poly(A) + RNA from porcine kidney cell line LLC-PK1. Pharmacological antagonism of the expressed V2 receptor was observed between arginine vasopressin and two potent and selective vasopressin antagonists: [d(CH 2 ) 5 , D-Phe 2 , Ile 4 , Ala 9 -NH 2 ]arginine vasopressin and [d(CH 2 ) 5 , D-Ile 2 , Ile 4 ]arginine vasopressin. Activation constant for arginine vasopressin concentration was 1.32 × 10 −10 M. The nucleotide length of the mRNA encoding for vasopressin V2 receptor was deduced to be approximately 2 kilobases.