Effects of caprylic acid and β-cyclodextrin complexes on digestibility, energy balance, and methane production in Korean Hanwoo heifers

Abstract The cyclodextrin (CD) complex with guest materials appears to be a promising solution to mitigate methane emissions without reducing energy intake. The purpose of this study was to determine the effects of caprylic acid (C8)-CD complex on methane production in vitro and in vivo . The C8-CD complex was added to diluted ruminal fluid (60 mL) at different levels (0, 0.17, 0.33, and 0.67 g/L) in triplicate and incubated anaerobically at 39 °C for 9 h. The pH (P  in vitro but not in vivo . Therefore, further research is required to determine whether higher supplementation of C8-CD complex than 2.0% of DMI reduce methane production in vivo .