RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND DEXAMETHASONE IN EYE/EAR DROPS
2013
High performance liquid chromatography method was applied to the simultaneous determination of ciprofloxacin and dexamethasone. The chromatographic separation was achieved on reversed-phase C18 column(25 cm × 4.6 mm id, 5 µm) in the isocratic mode using methanol-water-triethylamine (55:45:0.6, v/v/v), pH adjusted to 3.0 ± 0.05 with orthophosphoric acid as the mobile phase at a flow rate 0.8 ml/min. Quantitation was achieved with UV detection at 254 nm. In the HPLC method, quantification was achieved over the concentration range of 3-18 and 1-6µg/ml, with mean recoveries of 99.94 ± 1.51 and 100.28 ± 1.25% for ciprofloxacin and dexamethasone respectively. The proposed methods were successfully applied for the analysis of synthetic mixtures and pharmaceutical formulations of ciprofloxacin and dexamethasone without any interference from common excipients.
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