High Performance Liquid Chromatography (HPLC) Fingerprinting, Mineral Composition and In Vitro Antioxidant Activity of Methanol Leaf Extract of Synsepalum dulcificum (Sapotaceae)
2017
This study was carried out to
identify the phytochemicals and
in vitro
antioxidant activity of methanol leaf
extract of
Synsepalum dulcificum
(MSD). Standard protocols were used to evaluate the total phenols, total
flavonoids and total antioxidants content of the extract. Nitric oxide (NO), hydroxyl radical (OH), ABTS
·+
and
2
,2
-
diphenyl
-
1
-
picrylhydrazyl (DPPH) radicals scavenging activity, inhibition of lipid peroxidation and the
ability of MSD to chelate ferrous ion as well its reductive potential were also evaluated. High Performance
Liquid Chromatography
(
HPLC) was used to
confirm the presence of polyphenols and carotenoids. Results
showed the presence of flavonoids, saponins, terpenoids and cardiac glycosides in the extract. Among others,
appreciable levels of potassium, calcium, sodium and magnesium were detected in the e
xtract. The IC
50
of the
extract for DPPH, NO, OH
-
, and ABTS
·+
radicals scavenging assays were 139.45 μg/ml, 119.17 μg/ml, 147.65
μg/ml, and 135.83 μg/ml respectively
.
It could be inferred that MSD showed appreciable
in vitro
antioxidant
activity and could be useful in preventing and ameliorating diseases in which free radicals are implicated.
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