Genetype distribution of mycoplasma pneumoniae by denaturing high-performance liquid chromatography

Objective To investigate the genetype distribution of mycoplasma pneumoniae (MP) by denaturing high-performance liquid chromatography (DHPLC). Methods A total of 300 cases nasopharyngeal aspirate were collected from our hospital. The MP genes of standard strains and clinical specimens isolates were amplified by PCR followed by DHPLC and genetype determination. Results A total of 110 cases were positive after 24 hours fermentation from 300 cases with pharyngeal swab. By the specific primers of MP-129, MP-FH standard strain and specimens, 2 280 bp and 2 580 bp gene fragments were made out respectively. One hundred and ten strains of clinical isolates were detected by DHPLC. One hundred and seven strains of P1-Ⅰ were 1b subtype, 3 were type P1-Ⅱ which were all 2a subtype. Conclusion The genetype of MP infection in children from our hospital is P1-Ⅰ, 1b subtype by using DHPLC technology. Key words: Denaturing high-performance liquid chromatography; Mycoplasma pneumoniae; Genetype