A Study on the efficacy of Withaferin-A in reducing oxidative stress against DEN induced hepatocellular carcinoma

To evaluate the modulating efficacy of Withaferin-A (WFA) against N-nitrosodiethylamine (DEN)- induced hepatocellular carcinoma in male rats at the biochemical, oxidative stress and antioxidants levels. The animals were divided into five groups 6 rats each. Group I was served as control. Group II was treated with dose of DEN. The group III and IV were served as pre- and post-treated animals respectively. Groups V was serve drug control treated with Withaferin-A alone. Administration of DEN has brought down the levels of membrane bound enzymes and antioxidants; whereas increased level of ROS, free radicals and LPO, which were later found to be reversed by the administration of Withaferin-A. The findings from the above study suggest the effectiveness of Withaferin-A in reducing the oxidative stress mediated changes in the liver and improving the functions, antioxidant capacities of rats.