Mycobacterium tuberculosis Growth Inhibition by Constituents of Sapium haematospermum

Four novel compounds consisting of two new pimaranes, lecheronol A (1) and lecheronol B (2), an acylated cycloartane, 3-O-β-lauroyl-cycloart-(23E)-en-25-ol (10), and a highly oxygenated novel chalconoid, α,β,3,4,5,2',4',6'-octahydroxydihydrochalcone (12), were isolated along with seven known triterpene derivatives and three flavonol glucosides from Mycobacterium tuberculosis growth-inhibiting fractions of the CH 2 Cl 2 /MeOH (1:1) extract of the aerial parts of Sapium haematospermum. Compounds 1, 3 (3α-hydroxyolean-12-ene), 8 [3α-hydroxylup-20(29)-en], and 9 (cycloartanol) were found most active, with MIC values of 4, 12.2, 13.4, and 8 μg/mL, respectively. Cytotoxicity tests in Vero cells for compounds 1, 3, 8, and 9 gave IC 50 values of 104.8, 127.2, 127.2, and 102.4 μg/mL, respectively.