Screening and Identification of Receptor reacting with Nucleocapsid Protein of Duck Enteritis Virus

To clarify the pathogenesis of Duck enteritis virus(DEV),the cDNA library of duck's liver infected by DEV and a bait plasmid containing DEV nucleocapsid protein(NP) gene were constructed,then the receptor was screened from the cDNA library plasmid by the yeast two-hybrid system and verified by GST pull-down test.The results showed that the capacity of the primary cDNA library was 1×106 CFU with insertion size from 0.5 to 1 kb,and the bait plasmid of pGBKT7-NP showed no self-activation.The receptor reacting with DEV NP in duck liver was initially confirmed as the protein kinase C inhibitor(PKCI).These results provide new clues for further investigation on pathogenesis of DEV.