The effect of androgens on the urinary excretion of ketosteroids, nonketonic alcohols and estrogens.

INTRODUCTION THE development of a method for the colorimetric estimation of urinary steroid alcohols (3) and a fluorometric method for the estimation of urinary estrogens (2) makes it possible to carry out simultaneous studies of these components as well as ketosteroids in the urine of patients under various types of hormone therapy. This report will be concerned with the effect of the administration of dehydroepiandrosterone1 and testosterone on the urinary excretion of ketosteroids, steroid alcohols and fluorogenic phenols2 (FP) in a series of 6 patients with cancer of the breast (female), 1 patient with cancer of the prostate gland and 4 patients with rheumatoid arthritis.3 METHODS Twenty-four-hour urine specimens were collected during preliminary control periods, periods of therapy and post-treatment control periods. Daily determinations of creatinine were performed to check completeness of the collections. An aliquot of each 24-hour specimen was hydrolyzed by boiling for ten minutes with J 5 volumes ...