Rapid and simple DNA extraction method from rice using a glass-fiber filter inserted pipette tip

In recent years, DNA discrimination techniques have been applied to various aspects of plant breeding such as marker-assisted selection using DNA markers, cultivar identifications of the cultivars for the protection of the breeding rights and for the prevention from the contamination of undesirable cultivars, and the examination of the relationships between closely-related cultivar lines. These DNA discrimination techniques are based on the detection of polymorphisms in nucleotide sequences in genomic or organelle DNAs. In many cases, genotypic difference is identified by detecting the polymorphisms in various ways after amplifying a part of the genomic DNA with the PCR method. There are some important points for the extraction of template DNA used for the PCR method. First, it is not necessary to extract large size DNA molecules. Thus, we do not need to worry too much about the DNA cleavages. Next, polysaccharides and polyphenols contained in plant tissues act inhibitorily for the PCR reactions. This means that it is essential to remove these substances as much as possible. Also, a large number of samples have to be handled in many cases, such as line selection in plant breeding. Consequently, it is necessary to develop a new method which enables operational simplification and cost reduction.