Seasonal Variation of Total Flavonoids and Luteolin in Humulus scandens

Seasonal variation of total flavonoids and luteolin in Humulus scandens were investigated.The content of total flavonoids was determined by ultraviolet spectrophotometric method.The content of luteolin was measured by RP-HPLC.The contents of total flavonoids and luteolin in H.scandens in different months were significantly different.The trend of seasonal variation of total flavonoids and luteolin were similar.The content range of total flavonoids varied from 14.74 mg·g~(-1) to 23.83 mg·g~(-1).The contents of luteolin ranged from 0.25 mg·g~(-1) to 1.11 mg·g~(-1).The contents of total flavonoids and luteolin in the sample harvested in Octover were higher than others.