Prostaglandin-Mediated Stimulation of Adenosine 3′,5′- Monophosphate Accumulation and Parathyroid Hormone Release in Dispersed Human Parathyroid Cells

Freshly dispersed cells were employed to study the effects of various prostaglandins (PGs) on cAMP accumulation and parathyroid hormone release in abnormal human parathyroid tissue. PGE1 and PGE2 effected dramatic increases in intracellular cAMP accumulation over a concentration range of 10−6–10−4 M; the relative effectiveness of these agents varied among different preparations. PGF2α caused a smaller stimulation of cAMP accumulation, and PGF1α was generally without effect. In contrast with the effect previously described in the bovine parathyroid cell system, PGF2α did not suppress agonist agoniststimulated cAMP accumulation. Both PGE1 and PGE2 enhanced cellular release of parathyroid hormone, with dose-response characteristics similar to those seen with cAMP. In addition, both agents led to a significant stimulation of adenylate cyclase activity in a cellular homogenate preparation. Neither indomethacin (10−5 M) nor naproxen (10−4 M) altered the calcium suppressibility of the cells, suggesting that endo...