Cloning of GST Gene in Plant Expression Vector

Tau class glutathione transferases (GSTU) genes are plant specific, induced by different abiotic stresses and protect plants against oxidative stress. GST gene of Salicornia brachiata was made full length from the EST sequence using 5’ RACE. SbGST gene was cloned in pGEMT vector. SbGST gene was excised from pGEMT vector using restriction enzymes Kpn I and Xho I, further the gene was introduced into pRT101 expression vector. Full cassette containing CaMV35S constitutive promoter with SbGST gene was excised with restriction enzymes Pst I from pRT101 vector and cloned in pCAMBIA1301 vector. The pCAMBIA1301 vector containing SbGST gene was mobilized in Agrobacterium tumefaciens (LBA 4404) and transformed to tobacco (Nicotiana tabacum cv. xanthi) by leaf disk transformation method.