A kind of method of fast and low-cost screening protein expression condition

The present invention relates to a kind of methods of fast and low-cost screening protein expression condition, and steps are as follows: (1) being grouped and carry out grown cultures to expression bacterial strain, bacteria liquid sample before inducing is made；(2) Fiber differentiation is grouped and carried out again to bacteria liquid sample before inducing, bacteria liquid sample after induction is made；(3) it takes bacteria liquid sample before induction to be separated by solid-liquid separation with bacteria liquid sample after induction, thallus is resuspended, thallus re-suspension liquid is made；It takes thallus re-suspension liquid to mix with sample-loading buffer, detects, obtain A1 and B1；(4) thallus re-suspension liquid is taken to be uniformly mixed with lysate, ultrasonication, separation of solid and liquid detect after absorption, obtain C1；Solid after separation of solid and liquid is uniformly mixed with sample-loading buffer, and detection obtains D1；(5) it is analyzed by data, screening and culturing condition.The present invention is put into without expensive device, and experimental result is accurate, and culture medium dosage, culture bottle cleaning and equipment frequency of use is greatly reduced, greatly shortens Preparatory work of experiment time and interpretation of result time.