Determination of Anti-carcinoma Activity of Ribonucleic Acid II

Seven cell lines were treated with different concentrations of RNA Ⅱ. The cell proliferation was determined by WST-8 colorimetric assay, and the sensitive cell line, human leukemia cell line K562, was chosen as the experiment object. Experiment conditions including cell density and incubation time were investigated. It was linear for RNA Ⅱ in the range of 0.02- 100 mg/ml. The anti-carcinoma activities of 10 batches of samples were determined by this method, and their inhibition rates to K562 cells were all over 55%.