La utilidad del oligonucleótido biotinilado del gen gag h1gag1584 para la detección electroquímica sin mediador del ADN proviral del VIH-1
2021
The aim of the study is to demonstrate that the biotinylated 19 base oligonucleotide probe of the gag gene H1Gag1584 can be used as a molecular recognition element for the detection of HIV-1 proviral DNA, in two electrochemical detection systems of free laber. Two free leber electrochemical detection systems were evaluated: an impedimetric DNA genosensor using a self-assembled biotin monolayer (SAM) and a voltammetric DNA genosensor by modifying a new epoxy graphite transducer with graphene oxide. The results obtained in this study showed that the biotinylated 19-base oligonucleotide probe of the gag gene H1Gag1584 can be used as a molecular recognition element for the detection of HIV-1 proviral DNA, in two electrochemical detection systems of free labels.
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