A Simple and Sensitive Determination of Histamine and Nτ-Methylhistamine in Biological Fluids by High-Performance Liquid Chromatography with Electrochemical Detection
1987
The determination of picomolar levels of histamine and its major metabolite, Nτ-methylhistamine, in biological fluids was achieved using reversed-phase liquid chromatography coupled with electrochemical detection. A simple sample purification procedure for blood and urine samples was carried out prior to analysis using an Amberlite CG-50 cation-exchange resin, which afforded an excellent recovery of both compounds.
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