Thienylpyridines as a New Fluorescent Reagent. II. Determination of Carboxylic Acid with 5-(4-Pyridyl)-2-thiophenemethanol Using HPLC

The title compound has been prepared and used as a new derivatization reagent. The reagent reacts with the carboxylic acids in chloroform in the presence of 1-isopropyl-3-(3-dimethylaminopropyl)carbodiimide to produce the corresponding fluorescent esters, which can be separated on an octadecyl-silica column. Each 5 fmol of hexadecanoic or octadecanoic acids and about 50 fmol of benzoic and p-anisic acids (1 μl injection) (S/N=2) were detected.