Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase
1989
When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of {sup 3}H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of he label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at 1:1 ratio with molybdenum.
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