Evaluation the cytotoxic/viability effect of gelatin/bioactive glass conduits by MTT assay

Peripheral nerve injury can successfully heal,if an appropriate environment and route be provided.This study was designed to develop a novel gelatin/ bioactive glass conduits using freeze-drying technique. The gelatin/ bioactive glass conduits characterized using Fourier transform infrared spectroscopy (FTIR).The surface morphology of the Nanocompositewere investigated through scanning electron microscopy (SEM).Cytotoxicity Evaluation show that the CHO cells had attached and proliferated in the surroundings of the conduit. Biocomptibilitywas assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenylte-2H-tetrazolium bromide) assay which showed that gelatin/bioactive glass conduits had good cytocompatibilit.