PROTECTION OF QISHENYIQIWAN ON CARDIOMYOCYTE INJURY INDUCED BY HYDROGEN PEROXIDE IN VITRO

Objective: To study the protection of Qishenyiqiwan on cardiomyocyte injury induced by hydrogen peroxide in vitro. Method: Hydrogen dioxide injured model was implemented with purified cultured cardiomyocytes, and the experiment was divided into 5 groups with 8 pores in each group(24-well cell culture plate): normal control group, hydrogen peroxide(200 μmol·L-1 )injured group, three different Qishenyiqiwan dose(15, 30, 45 μg·L-1) groups. For Qishenyiqiwan groups, the cell culture was damaged by hydrogen peroxide while adding Qishenyiqiwan, and the incubation time was 12 h. The cell culture medium was used for assaying the activity of lactate dehydrogenase (LDH) , creatine phosphokinase (CPK), antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione-peroxidase (GPx), and glutathione (GSH). Cultured myocardial cells apoptosis was detected by flow cytometry. Results: Compared with the hydrogen peroxide injured group, the activities of marker enzymes, LDH, CPK in the cell culture medium of the normal control group and Qishengyiqiwan group were significantly decreased(P0.05), while the activities of SOD, CAT, GPx and GSH were significantly increased(P0.05), and apoptosis ratio was decreased markedly(P0.05). Conclusion: The present study reveals that Qishenyiqiwan can significantly reduce myocardial damaged by Hydrogen dioxide in vitro.