Studies on peptides. LIX. Synthesis of the nonatriacontapeptide corresponding to the entire amino acid sequence of porcine adrenocorticotropic hormone.

The nonatriacontapeptide corresponding to the newly revised amino acid sequence of porcine adrenocorticotropic hormone (ACTH) was synthesized by successive condensations of 4 peptide fragments ; Z (OMe)-(15-19)-OH, Z (OMe)-(11-14)-OH, Z (OMe)-(5-10)-OH and Z-(1-4)-NHNH3, with H-(20-39)-OBzl, a synthetic intermediate of porcine corticotropin-like intermediate lobe peptide. The synthetic peptide exhibited the identical Rf value with that of natural porcine ACTH in two different solvent systems and its in vivo steroidogenetic activity was 148.2 IU/mg.