Method for somatic cell nuclear transfer in zebrafish

Abstract Somatic cell nuclear transfer (SCNT) has been a well-known technique for dec- ades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elab- orated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish research community. In this chapter, we metic- ulously outline each step of the published protocol as well as preparations of equip- ments and reagents used in zebrafish SCNT. All describable detailed-tips are elaborated in texts and figures. Zebrafish has been increasingly utilized as avertebrate model for human diseases and developmental biology as it possesses advantageous characteristics, many of which are far better than other established animal models. As its uses widen among laboratories, the demand for sophisticated genetic manipulations only grows, i.e., conditional knock in, knockout, and knockdown among others. Many research groups have already established a wide variety of approaches for generation of both