[The effect of peroxiredoxin 2 on transforming growth factor-beta1-induced fibroblast proliferation and collagen synthesis].

Objective: To investigate the effect of peroxiredoxin 2 (Prx2) overexpression on fibroblast proliferation and collagen synthesis induced by transforming growth factor-beta1 (TGF-beta1) . Methods: Fibroblasts were randomly divided into control group (DMEM medium) , TGF-beta1 group (5 mug/L TGF-beta1) , negative control group (treated with 5 mug/L TGF-beta1 and transfected with empty lentiviral vector) , and Prx2 group (treated with 5 mug/L TGF-beta1 and transfected with Prx2 overexpression lentiviral vector) . MTT assay was used to measure cell proliferation, immunofluorescence assay was used to measure the expression of 8-OHdG, and Western blot was used to measure the expression of p-JNK, p-P38, collagen type I, collagen type III, and Prx2. SPSS 18.0 was used for statistical analysis. The continuous data were expressed as mean+/-standard deviation; an analysis of variance was used for comparison between groups, and the least significant difference t-test was used for further comparison between two groups. Results: Lentiviral transfection was performed successfully, and the Prx2 group had a significant increase in the protein expression of Prx2 (P 0.05) , while the Prx2 group had significant reductions in the above parameters (P<0.05) . Conclusion: Prx2 overexpression inhibits fibroblast proliferation and collagen synthesis induced by TGF-beta1 through inhibiting reactive oxygen species and activating the JNK and P38 pathways.