The protective effect of Tongxinluo to endothelium injured by angiotensin-II in vivo in rats

Objective:To observe the protective effect of Tongxinluo on artery endothelium injured by angiotensinⅡ osmotic pump in rat.Methods:54 SD rats were divided into sham group(18 rats),angiotensinⅡ group(18 rats) and Tongxinluo group(18 rats).There were three time points,7d,14d and 28d in each group.AngiotensinⅡosmotic pump at the dose of 200ng·min-1·kg-1 were embedded in the rat back of angiotensinⅡ(AngⅡ) group and Tongxinluo(TXL) group.In TXL group,Tongxinluo(0.8gng·kg-1·d-1) was given by gastric tube after AngiotensinⅡpump were used.All animals were sacrifi ced in 7th,14th and 28th day respectively.Blood pressure,AngⅡ,ET-1,6-Keto-PGF1α,Bax,Bcl-2 and p22phox mRNA were measured in different methods.Results:The plasma level of angiotensinⅡ in AngⅡ group and TXL group was higher than the Sham group,meanwhile the blood pressure measured from carotid artery of the three groups was not high.Compared with AngⅡ group,the blood level of 6-Keto-PGF1α,the Bax and NADPH p22phox mRNA signifi cantly decreased in TXL group,especially in the time point of 14d and 28d.The marked difference of the level of ET-1 between AngⅡ group and TXL group existed in the time point of 14d.The signifi cant difference of Bcl-2/Bax between AngⅡ group and TXL group existed in three time points.Conclusions:Tongxinluo could protect the endothelium against the injury from AngiotensinⅡby inhibiting the expression of p22phox mRNA,Bax mRNA and Bcl-2 mRNA and reducing the blood level of ET-1 and 6-Keto-PGF1α.