Serum Enzymes Derived from Liver Cell Fractions: II. Responses to bile duct ligation in rats

The activities of enzymes localized to liver cell cytoplasm, mitochondria, endoplasmic reticulum, nuclei, and lysosomes were measured in serum and subcellular fractions after bile duct ligation in rats. The patterns of response were compared to ' those occurring after acute CCl 4 injury. In the first 24 hr, marked increases occurred in serum sorbitol dehydrogenase (cytoplasmic) and ornithine carbamyl transferase (mitochondrial) activities, equal to those after CCl 4 . From 24 hr to 18 days, these activities fell to lower, but still elevated, levels. No increase in serum microsomal liver esterase occurred after bile duct ligation, whereas the activity was elevated 24 hr after CCl 4 . Neither serum arylsulfatase B (lysosomal) nor nicotinamide mononucleotide adenylyl transferase (nuclear) activities changed after duct ligation. No correlations between serum and cell fraction activities were observed.