Do the amount of Proteinase K and the incubation time of samples make a difference in DNA extraction from bones? A comparative study of protocols

Abstract In protocols of DNA extraction, the components of extraction buffers, Proteinase K and incubation time are important factors for the amount of DNA recovered and the quality of DNA profiles obtained. In this study, we compared two protocols of DNA extraction from bones using different amounts of Proteinase K and different incubation times in order to provide some data regarding higher amounts of Proteinase K and longer incubation time with the amount of DNA recovered and the quality of STR profiles obtained from twenty bone samples. The data provided in this study showed that higher amounts of Proteinase K and time of incubation longer than what is recommended in the standard protocols did not make a difference for the better in the extraction protocols tested.