Large scale preparation method of microbe microcapsules

The invention provides a method for large-scale preparation of micro-organism microcapsules, belonging to the technical field of micro-organism and biology. The method of the invention comprises the steps as follows: (1) capsule solution preparation: preparing the sodium alginate solution with the mass volume specific concentration of 1-2% w/v and carrying out the operation of sterilization; (2) preparation of curing agent: preparing the CaCl2 solution of 0.1-0.3mol/L and sterilizing; (3) preparation of the micro-organism microcapsules: micro-organism seed liquid with the logarithmic growth phase is added into the sterilization capsule solution till that the density of the solution is about 10 cfu/ml; the solution is then injected into the aseptic CaCl2 solution through the aseptic filter film and a nozzle by high-pressure air, then mixed and solidified till that supernatant is precipitated so as to gain the microcapsule and to wash the capsule; (4) after-covering culture: the microcapsules covered by micro-organism cells are arranged in fermentation broth and cultured till logarithm growth telophase, so as to gain the micro-organism microcapsule by filtration and separation. The method of the invention has the advantages of overcoming the defect that the micro-organism microcapsule which is prepared by covering-method before the sterilization can not be produced in large-scale, large production, simple operation and low cost of material and equipments.