Virus Detection in Biopsy Specimens

We describe nonradioactive in situ hybridization (ISH) approaches for localization of viral DNA and RNA sequences in infected tissue. The use of non-isotopic ISH methods has become common because they are now as sensitive as autoradiography but they do not have the the disadvantages of the isotopic methods, such as the long assay time and the requirement for special laboratory facilities (Musiani et al., 1994).