Hepatopathy detecting test paper by colloidal gold chromatography and preparation method thereof

The invention discloses hepatopathy detecting test paper by colloidal gold chromatography and a preparation method thereof. The hepatopathy detecting test paper by colloidal gold chromatography is a film tape formed by sequentially and mutually overlapping and pasting a pyroxylin coating film, a bonding pad, a sample pad and a water-absorption pad, wherein the pyroxylin film is provided with a detecting line and a quality control line, the detecting line coats M2 type mitochondrial antigenic protein, the quality control line coats an antibody (c), and the bonding pad coats a colloidal gold labeled antibody (a) and a colloidal gold labeled antibody (b). In the test paper tape provided by the invention, the M2 type mitochondrial antigenic protein is introduced, and process optimization is carried out on the bonding pad and the sample pad, thus realizing detection performance with high sensitivity, high specificity and high accuracy on the M2 type anti-mitochondrial antibody.