Dynamic aspects of extracellular loop region as a proton release pathway of bacteriorhodopsin studied by relaxation time measurements by solid state NMR

Abstract Local dynamics of interhelical loops in bacteriorhodopsin (bR), the extracellular BC, DE and FG, and cytoplasmic AB and CD loops, and helix B were determined on the basis of a variety of relaxation parameters for the resolved 13 C and 15 N signals of [1- 13 C]Tyr-, [ 15 N]Pro- and [1- 13 C]Val-, [ 15 N]Pro-labeled bR. Rotational echo double resonance (REDOR) filter experiments were used to assign [1- 13 C]Val-, [ 15 N]Pro signals to the specific residues in bR. The previous assignments of [1- 13 C]Val-labeled peaks, 172.9 or 171.1 ppm, to Val69 were revised: the assignment of peak, 172.1 ppm, to Val69 was made in view of the additional information of conformation-dependent 15 N chemical shifts of Pro bonded to Val in the presence of 13 C– 15 N correlation, although no assignment of peak is feasible for 13 C nuclei not bonded to Pro. 13 C or 15 N spin–lattice relaxation times ( T 1 ), spin–spin relaxation times under the condition of CP-MAS ( T 2 ), and cross relaxation times ( T CH and T NH ) for 13 C and 15 N nuclei and carbon or nitrogen-resolved, 1 H spin–lattice relaxation times in the rotating flame ( 1 H T 1ρ ) for the assigned signals were measured in [1- 13 C]Val-, [ 15 N]Pro-bR. It turned out that V69–P70 in the BC loop in the extracellular side has a rigid β-sheet in spite of longer loop and possesses large amplitude motions as revealed from 13 C and 15 N conformation-dependent chemical shifts and T 1 , T 2 , 1 H T 1ρ and cross relaxation times. In addition, breakage of the β-sheet structure in the BC loop was seen in bacterio-opsin (bO) in the absence of retinal.