Real-time PCR detection of relative expression of sBD-1 mRNA of epithelial cell of fallopian tubes in Mongolian sheep.

To detect the relative expression of sBD-1 mRNA in the cultured epithelial cell of fallopian tubes of Mongolian sheep,a real-time PCR with SYBR GreenⅠ was performed.The in vitro model of cultured system of fallopian tubes epithelial cell in Mongolian sheep was established,and the total RNA of epithelial cell was extracted.With β-Actin gene as a reference gene,a real-time PCR with SYBR GreenⅠ was carried out.The amplified product was confirmed to be β-defensins gene by sequencing.The results showed that the relative expression of sBD-1-mRNA in the cultured epithelial cells of Mongolian sheep could be detected using the real-time PCR with SYBR GreenⅠ.