Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4

The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapour- diffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 A resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 A, β = 112.99° and three molecule per asymmetric unit.