Biological investigations of the ethanol extract of the aerial part (leaf) of Coccinia grandis L

In this present study, the leaf extracts of Coccinia grandis L., were subjected to evaluation of the phytochemical screenings, thrombolytic, membrane stabilizing, antioxidant, antimicrobial and cytotoxic activity. The ethanol extract showed 43.71% clot lysis as compared to 65.16% clot lysis produced by standard streptokinase. In vitro membrane stabilizing activity for hypotonic solution and heat induced haemolysis the ethanol extract inhibited 79.53% and 90.30% haemolysis of RBCs as compared to 71.90% and 77.20% produced by acetyl salicylic acid respectively. In vitro antioxidant activity revealed that the ethanol extracts of C. grandis exhibited significant antioxidant activity with IC50 value of 1.15 mg/ml in comparison with the standard 5.80 mg/ml. Leave extracts of Coccinia grandis L. revealed moderate antimicrobial activity against some gram positive and gram negative microorganisms. Leave extracts showed strong inhibitory effect on brine shrimp lethality with LC50 at 24.20 μg/ml.