EGCG对鼻咽癌CNE-2细胞增殖、凋亡的影响及机制

Objective To explore the anti-cancer effect and mechanism of(-)-Epigallocatechin-3-Gallate (EGCG) on nasopharyngeal carcinoma. Methods Nasopharyngeal carcinoma cell line CNE-2 were cultured in vitro and treated with various concentrations of EGCG, the proliferation inhibition rate was detected by MTT, the apoptosis was analyzed by flow cytometry, the expression of MnSOD was detected by RT-PCR. Results The CNE-2 proliferation was inhibited in a dose-and time-dependent manner after treated by EGCG, the CNE-2 apoptosis ratio increased significantly in dose-dependent manner. The expression of MnSOD increased significantly in time-dependent manner. Conclusions EGCG can suppress the proliferation and induce apoptosis in CNE-2, possible mechanism is up-regulating the expression of MnSOD. This study may provide a theoretical basis for the prevention and therapy of nasopharyngeal carcinoma by EGCG.