Bovine dialyzable leukocyte extract modulates cytokines and nitric oxide production in lipopolysaccharide-stimulated human blood cells

BackgroundIn the current study, we determined whether bovine dialyzable leukocyte extract (bDLE) modulates lipopolysaccharide (LPS)-induced nitric oxide and cytokine overproduction.MethodsHuman whole blood cells were treated with LPS (50 ng) + bDLE (1 U).ResultsThe bDLE treatment decreased nitric oxide as well as TNF-α, IL-6 and IL-10 (P <0.01) cytokine production. In addition, it decreased TNF-α, IL-1β and IL-6 mRNA expression and suppressed IL-10 and IL-12p40 mRNA expression, but did not modulate IL-8 mRNA expression in LPS-stimulated human blood cells.DiscussionOur results suggest that bDLE may effectively modulate the fatal symptoms of hypotensive shock associated with endotoxin (LPS)-induced nitric oxide and cytokine production, and this may offer therapeutic potential for the treatment of endotoxic shock.