The Culturing of SD Rat Costochondral Chondrocyte and Observation of Dedifferentiation

Objective To establish the separating and culturing methods for rat costochondral chondrocyte(RCC),and to investigate their morphous changes in the course of cell passage.Methods Under the help of collagenaseⅡand trypsin's digestion,the scattered single RCC was obtained.The observation of morphological changes of RCC cells cultured by monolayer culture in different generations was carried out.And the detection of the expression of collagen II and proteoglycan of RCC cells in different generations by immunocytochemical method was also implemented.Results High purity cartilage cells were obtained from rat costal cartilages after digested by collagenaseⅡand trypsin.It was confirmed by immunohistochemistry and toluidine blue staining.The dedifferentiation of RCC cells was observed when the RCC cells were cultured by monolayer culture.Conclusions RCC cells can be amplified by passage Culture,but only within the cells of four generations.