Cryoprotectant Effect of Trehalose on Frozen-Thawed Boar Sperm

The objective of this study was to investigate the influence of the addition of cryoprotectants to freezing extenders on post thawing boar sperm quality as well as investigate their optimum concentrations. To evaluate the effect of trehalose (0, 80, 100, 120 mM) added in egg yolk extender freezing medium on motion characteristics, motility, viability and DNA integrity of spermatozoa at post thawing of cryopreservation. Semen were collected from 10 boars and frozen on the same day. Qualified semen samples (motility >80%) from each boar were subdivided four groups, 0 (control), 80 mM (T1), 100 mM (T2) and 12 0 mM (T3). Motility was assessed for % motile cell characteristics using computer-assisted semen analysis (CASA; SAIS SI-100, Medical supply, Korea). Frozen boar sperms were thawed in Beltsville Thawing Solution (BTS) then incubated at 38 celsius degree for 20 minutes. Computer progressive motility, average path velocity, straight line velocity, curvilinear velocity and total motility were higher (p<0.05) in adding 120 mM treatment group (T3) as compared with control. Moreover, trehalose addition did not damage DNA integrity of spermatozoa. Nevertheless, trehalose concentration treatment did not affect the viability of the spermatozoa as the concentration increased. The data showed that trehalose as an additive cryoprotectant was able to improve sperm quality without on sperm DNA integrity in cryopreservation.