수치방법(修治方法)에 따른 반하(半夏) 추출물(抽出物)의 마우스 단회투여독성실험(單回投與毒性實驗)

Objective : The object of this study was to evaluate the single dose toxicity of Pinelliae Rhizoma(PR) according to processing methods in ICR mice. Methods : In order to observe the change of acute toxicity of PR according to the processing methods, Aqueous extracts of three different processing PR were administered to female and male ICR mice at dose levels of 2000 ㎎/㎏ (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines (2009-116, 2009). The mortality and changes on body weight, clinical signs and gross observation were monitored during 14 days after dosing, histopathological observations of 12 types of principle organs were examined. In addition, the changes on the serum biochemistry related to the hepatic damages - serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin and alkaline phosphatase (ALP) were also observed. Results : After single oral treatment of three different PR aqueous extracts, we could not find any mortality and toxicological evidences to 2000 ㎎/㎏ in both female and male mice, No remarkable changes on the serum AST, ALT, albumin and ALP levels were also demonstrated in this study. Conclusion : The results obtained in this study suggest that the toxicity of PR were also reduced by all three different processing methods used in this study. There were no evidences indicating the hepatic damages as well. However, previous study about the not processing PR showed hepatic toxicities. Therefore, it is recommended that the processing PR should be used in clinics as like as suggestions of traditional methods.