The nucleotide sequence of the 5′ flanking region of the Arabidopsis ACS2 gene

The ACS2 gene of Arabidopsis thaliana encodes 1-aminocyclo-propane-1-carboxylic acid (ACC) synthase (EC 4.1.1.14) - the key regulatory enzyme in the biosynthetic pathway of the plant hormone ethylene. The gene is unique among ACC synthase genes isolated thus far in that it is highly expressed in both reproductive (e.g. flower) and vegetative tissues (e.g. leaves and roots). In addition, the accumulation of ACS2 transcripts is greatly induced by protein synthesis inhibition (Liang ef al 1992). We report here the DNA sequence of the 5′ flanking region of the ACS2 gene and an analysis of the sequence with respect to the potential c/s-acting regulatory elements that can be recognized by DNA-binding proteins from plants and other organisms.