Flavobacterium cheniae sp. nov., isolated from sediment of a eutrophic reservoir

A Gram-negative, rod-shaped, yellow pigmented bacterium, strain NJ-26T, was isolated from sediment of the eutrophicated Guanting Reservoir in Beijing, China. A phylogenetic analysis based on 16S rRNA gene sequences placed strain NJ-26T within the genus Flavobacterium in the family Flavobacteriaceae. The highest sequence similarity was found with Flavobacterium cucumis R2A45-3T (97.7 %). The major fatty acids (>5 %) of the isolate were iso-C15 : 0, iso-C17 : 1 ω9c, C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. The G+C content of the genomic DNA was 40.6 mol%. The DNA–DNA relatedness value with F. cucumis R2A45-3T was 5.4 %. Molecular and phenotypic data suggest that strain NJ-26T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium cheniae sp. nov. is proposed. The type strain is NJ-26T (=CGMCC 1.6844T =NBRC 103934T).