Analysis of monitor bacterial diversity in fermentive corn silage and alfalfa silage by PCR-DGGE method.

Changes in the composition of bacterial community in the corn silage and the alfalfa silage during the whole fermentation process were compared by using PCR-DGGE method.The results showed that the bacterial community in the alfalfa silage remained stable during the whole process.No clear bands disappeared or appeared on DGGE gel.The similarity indexes of the DGGE profiles during the whole process was over 79%.However,the bacterial community in the corn silage became clearly simple after 3 days fermentation.The similarity indexes of the DGGE profiles from 0 to 3 days and those from 7 to 60 days were less than 50%.The predominant bacteria of natural silage were originated from normal bacterial community of the original materials.In the subsequent experiment,19 bands of agarose gel which contained target sequences were recycled,and 15 bands were successfully cloned,including ?-Lactococcus,?-Acinetobacter,?-Streptococcus,?-Weissella,?-Pantoea,?-Escherichia,?-Enterobacter,?-Endophytic bacterium and so on.