Effect of high dry, wet temperatures and thermophilic, mesophilic methanic fermentation on the viability of Cryptosporidium parvum oocysts isolated from bovine faeces.

Cryptosporidium parvum oocysts isolated from bovine faeces were submitted to high dry, wet temperatures and thermophilic, mesophilic methanic fermentation in order to check their viability and resistance. The study showed that high dry temperatures destroy completely Cryptosporidium parvum oocysts after 45mn of exposure at 45 and 50°C, 35mn at 55°C, 25 mn at 60°C, 15mn at 65°C, 5mn at 70°C, 3mn at 74 and 80°C, 2mn at 85, 90 and 95°C. The high wet temperatures killed cryptosporidia oocysts after 60mn of incubation at 50°C, 30mn at 55°C and 5mn at 60 and 65°C. However, the hermophilic methanic fermentation was effective against Cryptosporidia oocysts after 40 and 20mn at 50 and 55°C respectively and the mesophilic after 8 days at 35°C. No alteration of morphology oocysts was observed even when the latest was exposed to higher temperatures (90 and 95°C).