Psychrobacter oceani sp. nov., isolated from marine sediment

A novel marine bacterium, designated strain 4k5T, was isolated from a sediment sample of the Pacific Ocean. The strain was Gram-stain-negative, strictly aerobic, non-motile, oxidase-positive and catalase-positive and required Na+ for growth. Its major isoprenoid quinone was ubiquinone 8 (Q-8), and its cellular fatty acid profile consisted mainly of C18 : 1v9c (71.4 %), C16 : 1v7c (9.1 %) and C18 : 0. The DNA G+C content was 45.3 mol%. 16S rRNA gene sequence analysis suggested that strain 4k5T is a member of the genus Psychrobacter . Strain 4k5T exhibited the closely phylogenetic affinity to Psychrobacter pacificensis IFO 16270T (99.4 % 16S rRNA gene sequence similarity), P. piscatorii T-3-2T (97.7 %), P. nivimaris 88/2-7T (97.7 %), P. celer SW-238T (97.7 %), P. aestuarii SC35T (97.6 %) and P. vallis CMS39T (97.6 %). DNA–DNA hybridization between strain 4k5T and P. pacificensis NBRC 103191T, P. piscatorii JCM 15603T. P. nivimaris DSM 16093T, P. celer JCM 12601T, P. aestuarii JCM 16343T and P. vallis DSM 15337T was 42.5, 47.0, 38.1, 23.7, 9.0 and 27.4 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on the 16S rRNA gene sequence and DNA–DNA relatedness data, the isolate merits classification within a novel species, for which the name Psychrobacter oceani sp. nov. is proposed. The type strain is 4k5T ( = JCM 30235T = NCIMB 14948T).