The study of combine mediated livin and survivin genes silencing induced proliferate inhibition and apoptosis in human renal carcinoma cell line 786-0

Objective To investigate the effect of combine mediated Livin and Survivin genes silencing induced the proliferation inhibition and apoptosis in human renal carcinoma cell line 786-0.Metheds Application of real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) and Western blotting methods respectively,to detect the expression level of Livin and Survivin in the renal cancer cell lines and tissues.Constructed Livin and Survivin combine targeting small interfering RNA (siRNA) recombinant expression vector,then transfected it into 786-0 cell line and validation.Application of the cell counting kit-8 (CCK-8) test and flow cytometer to investigate the proliferation inhibition and apoptosis of 786-0.Results The cell survival rate of the group of combine mediated Livin and Survivin genes silencing was significant decrease and then the cell apoptotic rate was significant increase,the proliferation inhibition enhanced 23％,26％ and apoptosis increased 100％,116％,respectively.Conclusion siRNA combine mediated Livin and Survivin gene expression,give play to the role of synergistic enhancement of siRNA. Key words: Small interfering RNA ;  Livin ;  Survivin ;  Renal carcinoma;  Proliferation inhibiting