STUDIES OF FIBRONECTIN-CANCER CELLS INTERACTIONS UNDER STATIC AND DYNAMIC CONDITIONS. DIFFERENT ADHESIVE BEHAVIOR OF HIGH METASTATIC AND LOW METASTATIC SUBCLONE OF NCI-H460

Metastatic tumor spread is pathologic process with series of adhesion/de-adhesion events. In this context, adhesion to, and detachment from the components of the ECM could be important measure for the invasive potential of cancer cells. Low (NCI-H460) and high (H-460-M2) metastatic lung carcinoma cell line were used to study the rates of adhesion to fibronectin under static and dynamic conditions. To measure the critical share force resulting in detachment of cancer cells from the protein-coated substrata, a special flow chamber was applied. Morphological immunofluorescence studies have been performed to better characterize the cellular adhesive interactions in static conditions. Thus, we found correlation between adhesive behavior and metastatic potential of cancer cells in respect of fibronectin in static and dynamic conditions: (i) In static condition both cell lines have the highest affinity to fibronectin in comparison to other matrix proteins