Method for preparing bioethanol through green tide algae enteromorpha in enzymolysis mode

2013 
The present invention relates to a process for the preparation of bioethanol, particularly to a preparation of green tide algae Enteromorpha bioethanol enzymatic methods, methods of preparation, Enteromorpha first washed, dried and crushed to 60-100 mesh, was added Lemon Enteromorpha acid buffer solution to obtain a mixture; cerevisiae Saccharomyces cerevisiae A and B were used to develop wort Jipei culture of Saccharomyces cerevisiae, and Saccharomyces cerevisiae A seed liquid seed liquid B; Enteromorpha then added to the mixture of cellulase and half cellulases, mixed, hydrolysis at 45-55 ℃ 40-60 hours to obtain a hydrolyzate; 5-10% of inoculum was added Saccharomyces cerevisiae and Saccharomyces cerevisiae a seed liquid B in the volume ratio of hydrolyzate seed liquid fermentation. The present invention, cellulase and hemicellulase synergistic effect, so that the sugar content of Enteromorpha significantly increased, improves the yield of bio-ethanol. The present invention is less demanding on the equipment, mild reaction conditions, simple process and environmental pollution.
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