[Cloning and expression in Escherichia coli of secondary lymphoid-tissue chemokine (SLC) gene].

Secondary lymphoid-tissue chemokine(SLC)is a type of CC chemokin e identified by searching the Expressed Sequence Tag(EST)database.The full-le n gth SLC gene was synthesized based on human SLC sequence using SOE-PCR.The s equenced SLC gene was cloned into expression vector pTMF and pALM,which used to transform Escherichia coli.Then the E.coli was cultured and induced ac cording to protocol.The expressed target protein was identified by Western blotting.Th e target protein was expressed as soluble protein as well as inclusion bodies,t he ratio of these two forms target protein varied with the difference conditions of culture and induction.The target protein was purified with the methods of n ickel-nitrilotriacetic acid(Ni-NTA)metal-affinity chromatography.The result s o f electrophoresis of the purified target protein showed that the molecular weigh t was larger than the predicted molecular weight.