Nucleotide sequence of the 3' terminal region of plum pox potyvirus RNA

Abstract The nucleotide sequence of the 3' terminal 2854 nucleotides of plum pox virus RNA has been determined. There is an open reading frame of 2634 nucleotides upstream from a 220 nucleotide 3' non-coding region followed by a long poly(A) tail. The gene coding for the capsid protein has been mapped adjacent to the 3' non-coding region. The predicted capsid protein is originated by a Gln-Ala cleavage and consists of 330 amino acids; the central and carboxy terminal, but not the amino terminal regions present high sequence similarity with other potyviral capsid proteins. A truncated capsid protein which is present in purified preparations of plum pox virus is originated by a Gln-Thr cleavage and contains the 260 carboxy terminal amino acids of the capsid protein. The predicted plum pox virus RNA-dependent RNA polymerase, 518 amino acids long, has been localized at the 5' end of the open reading frame.